Separating Charged Molecules For Identifying A Wide Range...

Cassandra Maddux CHE 451L-02 August 26, 2014 Electrophoresis Introduction Separating charged molecules is an important practice in Biochemistry for identifying a wide range of data fields. Separating molecules allows for information like size, binding affinity, and charge to be obtained.5 One technique that is used to separate charge molecules is gel electrophoresis. This technique forces the suspended charged molecules through a porous gel matrix by use of an electrical current that separates the molecules according to their physical properties, such as charge, size.1,2,3 It was first observed by Ferdinand Frederic Reuss in 1807. He observed that when an electrical current was applied, clay molecules in water would begin to migrate.6 Samples are placed in wells on the gel. A buffer is added, commonly salt water, to act as a conduit for the electrical current. As the electric current is applied, the samples begin to move through the gel depending on the contained molecule’s properties; positively charged particles will move towards the cathode and negatively charged particles will move to the anode.2 Compounds with greater charge or low mass will move through the gel matrix quicker and further. As the samples move, they create lanes of bands that can then be compared to a standard, also known at a ladder. The thickness of each band is indicates quantity of each compound contained in the sample. The height of each band shows the size of each molecule in the sample.4Show MoreRelatedSexually Transmitted Diseases35655 Words   |  143 Pagesmethod consists of the collection of data through observation and experimentation, and the formulation and testing of hypotheses.Scientific researchers propose hypotheses as explanations of phenomena, and design experimentalstudies to test these hypotheses. Steps in the Scientific Method 1. Define the question 2. Gather information and resources (observe) 3. Form hypothesis 4. Perform experiment and collect data 5. Analyze data 6. Interpret data and draw conclusions that serveRead MoreCase Study148348 Words   |  594 PagesLimited 2011 Lecturers adopting the main text are permitted to download and photocopy the manual as required. Pearson Education Limited Edinburgh Gate Harlow Essex CM20 2JE England and Associated Companies around the world. Visit us on the World Wide Web at: www.pearsoned.co.uk ---------------------------------This edition published 2011  © Pearson Education Limited 2011 The rights of Gerry Johnson, Richard Whittington and Kevan Scholes to be identified as the authors of this work have been assertedRead MoreExploring Corporate Strategy - Case164366 Words   |  658 Pagesstrategies and planning for the future in the wake of a global shock. Amazon (A) – long term planning of a successful dot.com. Jordan – the challenge of building capabilities for success in Formula 1. Shefï ¬ eld Theatres – strategy formulation for a wide audience of public and commercial stakeholders. Fisons – disastrous consequences of stakeholder management. Iona – Mission-driven strategy and stakeholder management. HomeCo – wrestling with governance and strategy in the boar droom; a role play. BMWRead MoreDeveloping Management Skills404131 Words   |  1617 Pagestheir products are claimed as trademarks. Where those designations appear in this book, and the publisher was aware of a trademark claim, the designations have been printed in initial caps or all caps. Library of Congress Cataloging-in-Publication Data Whetten, David A. (David Allred) Developing management skills /David A. Whetten, Kim S. Cameron.—8th ed. p. cm. Includes bibliographical references and index. ISBN 978-0-13-612100-8 1. Management—-Study and teaching. 2. Management—Problems, exercises